Common specifications

Spatial XYZ resolution of CARS signals
(Objective lens: 60х, NA=1.2, watter immersion):
< 0.7 μm
Spatial resolution of Raman signals
(excitation wavelength 633 nm; objective lens: 60х, NA=1.2, watter immersion):
XY: < 300 nm
Z:   < 700 nm
Spectral detectable range:

CARS signals:            985 – 5000 cm-1
Raman signals:          75 – 6000 cm-1

Spectral resolution: CARS signals:                7-8 cm-1
Raman signals:              0.25 cm-1(grating 75 l/mm Echelle);
                                    0.6 cm-1 (grating 1800 g/mm)
Scanning range (fast scanning mode, 60x lens) XY: 225 х 225 μm
Z:   80 μm
Control and automation: Fully motorized

Optical microscope 

Type: inverted
Model: Nikon Ti-U
Stage: motorized
- travel range: 114 х 75 mm
- accuracy (for 1 mm traveling): 0.06 μm
- XY repeatability: ± 1 μm
- minimal step: 0.02 μm
Micro- objective lens: 60 х NA-1.2 water immersion
20 x NA-0.45
- type: piezo scanner
- lens movement range: 80 μm
- minimal step: 50 nm
- Reapetability: < 6 nm
Illuminator for reflection mode: Halogen lamp 100 W
Illuminator for transmission mode: LED
Laser input port: motorized triple turret
High resolution digital video camera:  
- type: digital color CCD camera
- sensor: 1/2", 2048 x 1536 pixeles
- ADC: 10 bit,  12 frames per min

CARS excitation laser system

Single module optical parametric laser system consisting of:  
Picosecond solit state Nd:YVO4 laser  
Output wavelength: 1064 nm
Pulse duration: 6.5 ps
Output power: 6 W
Pulse repetition rate: 85 MHz
Mode composition: TEM00
Beam quality (М2): < 1.5
Beam diameter: 2 mm
Tunable SOPO
(Synchronously pumped Optical Parametrical Oscillator)
Wavelength tuning range: 690 - 990 nm
Output power: > 400 mW (at 800 nm)
Pulse duration: 5 - 6 ps
Pulse repetition rate: 85 MHz
Polarization: horizontal
Mode composition: TEM00
Beam quality (М2): < 1.2
Beam diameter: 2 mm
Built-in delay tuning optical line  


Laser for Raman spectroscopy

Laser type: cw HeNe
Wavelength: 632.8 nm
Average output power: > 10 mW
Polarization: linear
Beam diameter: 0.65 mm
Beam divergence: < 1.24 mrad

Laser beam convergence and appointment unit

Laser shutter: 3 pieces (Nd:YVO4 laser, SOPO and HeNe laser)
Polarizer: 2 pieces (Nd:YVO4 laser and SOPO)
Wavelength plate λ/2 2 pieces (Nd:YVO4 laser and HeNe laser)
Variable telescope: 3 pices (Nd:YVO4 laser, SOPO and HeNe laser)

Main optical unit

Optimized optics for spectral range: 400 - 1100 nm
Polarization analyzer: Glan-Teylor prism
Laser beam attenuator: veriable neutral density filter 0 - 3D
Pinhole lens positioner: three-axis (X, Y, Z)
CARS, Raman, fluorescence filters positioner: five- position
Posotioner of dihroic mirrors: six- position

Imaging monochromator / spectrograph MS5004i

Focal length: 520 mm
F number (input): 9.8
Horizontal magnification: 1.0
Vertical magnification: 1.0
Vertical spatial resolution: < 20 μm
Flat field size: 28 х 5 mm
Stray light
( 20 nm from 633 nm laser line)
1 х 10-5
Difraction gratings unit: 4-position motorized grating turret
Spectral resolution
(wavelength 633 nm, CCD pixel size: 12x12 μm)

0.01 nm (Echelle grating 75 l/mm)
0.025 nm (grating 1800 l/mm)

Entrance spectral slit motorized confocal pinhole,
continuously adjustable from 0 to 1.5 mm
Output spectral slit: motorized, width from 0 to 2 mm (continuously adjustable)
Ports: 1 input, 2 output
Detector (Raman, E-CARS, fast measurements) Hamamatsu Photosensor module H7844 (with TE cooling)

Monochromator for fluorescence measurements (integrated with MS5004i)

Focal length: 100 mm
Input spectral slit: motorized confocal pinhole
Output spectral slit: fixed, width 4.2 mm
Difraction grating: 600 l/mm
Spectral range: 400 - 920 nm
Linear reciprocal dispersion: 13 nm/mm
Detector Hamamatsu Photosensor module H7844 (with TE cooling)

Spectral CCD camera (Raman, E-CARS)

Type: digital CCD camera
Photosensor: back-thinned CCD sensor 2048 х 122 pixels
Pixel size: 12 х 12 μm
Photosensitive area: 24.576 х 1.464 mm (length х heigh)
Spectral sensitivity range: from 200 to 1100 nm
Cooling with temperature stabilization: two-stage Peltier (TE) cooling down to – 45 °С
ADC digitization: 16 bit
Sensitivity: 1 photon for 1 ADC count (at 650 nm)
Dinamic range: at least 10 000

Fast scanning unit (X, Y)

Scanners: Galvano-mirror scanners (X, Y)
Scanning model: raster high speed and start-stop mode
Positioning accuracy: < 30 μm
Scanning area: 225 μm х 225 μm (objective lens 60Х)
Full frame scanning speed: 4 s/frame: 1000 х 1000 points

Laser confocal microscope unit (Reflected)

Prepinhole lens positioner: three -axis (X, Y, Z)
Confocal pinhole: motorized confocal pinhole,
continuously adjustable from 0 to 1.5 mm
Detector: Hamamatsu Photosensor module H6780-01

F-CARS and transmitted signal registration system

Polarization alanyzer: Glan-Teylor prism
F-CARS filters positioner: 4-position, motorized
F-CARS signal registration detector: Hamamatsu Photosensor module H7844 (with TE cooling)
Transmitted signal registration detector: Hamamatsu Photosensor module H6780-01

Registration and processing control unit

Number of registration channels: 5
Number of simultaneously registered channels: up to 5
PC connection interface: Ethernet 100 Base-T (TCP/IP protocol)

Optical table

All components of the system based on the optical plate with the pneumatic vibrated insulating legs.