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Spatial XYZ resolution of CARS signals (Objective lens: 60х, NA=1.2, water immersion): |
< 0.7 μm |
|
Spatial resolution of Raman signals (excitation wavelength 633 nm; objective lens: 60х, NA=1.2, watter immersion): |
XY: < 300 nm Z: < 700 nm |
| Spectral detectable range: |
CARS signals: 985 – 5000 cm-1 |
| Spectral resolution: |
CARS signals: 7-8 cm-1 Raman signals: 0.25 cm-1(grating 75 l/mm Echelle); 0.6 cm-1 (grating 1800 g/mm) |
| Scanning range (fast scanning mode, 60x lens) |
XY: 225 х 225 μm Z: 80 μm |
| Control and automation: | Fully motorized |
| Type: | inverted |
| Model: | Nikon Ti-U |
| Stage: | motorized |
| - travel range: | 114 х 75 mm |
| - accuracy (for 1 mm traveling): | 0.06 μm |
| - XY repeatability: | ± 1 μm |
| - minimal step: | 0.02 μm |
| Micro- objective lens: |
60 х NA-1.2 water immersion 20 x NA-0.45 |
| Z-scanner: | |
| - type: | piezo scanner |
| - lens movement range: | 80 μm |
| - minimal step: | 50 nm |
| - repeatability: | < 6 nm |
| Illuminator for reflection mode: | Halogen lamp 100 W |
| Illuminator for transmission mode: | LED |
| Laser input port: | motorized triple turret |
| High resolution digital video camera: | |
| - type: | digital color CCD camera |
| - sensor: | 1/2", 2048 x 1536 pixeles |
| - ADC: | 10 bit, 12 frames per min |
| Single module optical parametric laser system consisting of: | |
| Picosecond solid state Nd:YVO4 laser | |
| Output wavelength: | 1064 nm |
| Pulse duration: | 6.5 ps |
| Output power: | 6 W |
| Pulse repetition rate: | 85 MHz |
| Mode composition: | TEM00 |
| Beam quality (М2): | < 1.5 |
| Beam diameter: | 2 mm |
|
Tunable SOPO (Synchronously pumped Optical Parametrical Oscillator) |
|
| Wavelength tuning range: | 690 - 990 nm |
| Output power: | > 400 mW (at 800 nm) |
| Pulse duration: | 5 - 6 ps |
| Pulse repetition rate: | 85 MHz |
| Polarization: | horizontal |
| Mode composition: | TEM00 |
| Beam quality (М2): | < 1.2 |
| Beam diameter: | 2 mm |
| Built-in delay tuning optical line |
| Laser type: | cw HeNe |
| Wavelength: | 632.8 nm |
| Average output power: | > 10 mW |
| Polarization: | linear |
| Beam diameter: | 0.65 mm |
| Beam divergence: | < 1.24 mrad |
| Laser shutter: | 3 pieces (Nd:YVO4 laser, SOPO and HeNe laser) |
| Polarizer: | 2 pieces (Nd:YVO4 laser and SOPO) |
| Wavelength plate λ/2 | 2 pieces (Nd:YVO4 laser and HeNe laser) |
| Variable telescope: | 3 pices (Nd:YVO4 laser, SOPO and HeNe laser) |
| Optimized optics for spectral range: | 400 - 1100 nm |
| Polarization analyzer: | Glan-Taylor prism |
| Laser beam attenuator: | veriable neutral density filter 0 - 3D |
| Pinhole lens positioner: | three-axis (X, Y, Z) |
| CARS, Raman, fluorescence filters positioner: | five- position |
| Posotioner of dihroic mirrors: | six- position |
| Focal length: | 520 mm |
| F number (input): | 9.8 |
| Horizontal magnification: | 1.0 |
| Vertical magnification: | 1.0 |
| Vertical spatial resolution: | < 20 μm |
| Flat field size: | 28 х 5 mm |
|
Stray light ( 20 nm from 633 nm laser line) |
1 х 10-5 |
| Difraction gratings unit: | 4-position motorized grating turret |
|
Spectral resolution (wavelength 633 nm, CCD pixel size: 12x12 μm) |
0.01 nm (Echelle grating 75 l/mm) |
| Entrance spectral slit |
motorized confocal pinhole, continuously adjustable from 0 to 1.5 mm |
| Output spectral slit: | motorized, width from 0 to 2 mm (continuously adjustable) |
| Ports: | 1 input, 2 output |
| Detector (Raman, E-CARS, fast measurements) | Hamamatsu Photosensor module H7844 (with TE cooling) |
| Focal length: | 100 mm |
| Input spectral slit: | motorized confocal pinhole |
| Output spectral slit: | fixed, width 4.2 mm |
| Difraction grating: | 600 l/mm |
| Spectral range: | 400 - 920 nm |
| Linear reciprocal dispersion: | 13 nm/mm |
| Detector | Hamamatsu Photosensor module H7844 (with TE cooling) |
| Type: | digital CCD camera |
| Photosensor: | back-thinned CCD sensor 2048 х 122 pixels |
| Pixel size: | 12 х 12 μm |
| Photosensitive area: | 24.576 х 1.464 mm (length х heigh) |
| Spectral sensitivity range: | from 200 to 1100 nm |
| Cooling with temperature stabilization: | two-stage Peltier (TE) cooling down to – 45 °С |
| ADC digitization: | 16 bit |
| Sensitivity: | 1 photon for 1 ADC count (at 650 nm) |
| Dinamic range: | at least 10 000 |
| Scanners: | Galvano-mirror scanners (X, Y) |
| Scanning model: | raster high speed and start-stop mode |
| Positioning accuracy: | < 30 μm |
| Scanning area: | 225 μm х 225 μm (objective lens 60Х) |
| Full frame scanning speed: | 4 s/frame: 1000 х 1000 points |
| Prepinhole lens positioner: | three -axis (X, Y, Z) |
| Confocal pinhole: |
motorized confocal pinhole, continuously adjustable from 0 to 1.5 mm |
| Detector: | Hamamatsu Photosensor module H6780-01 |
| Polarization analyzer: | Glan-Taylor prism |
| F-CARS filters positioner: | 4-position, motorized |
| F-CARS signal registration detector: | Hamamatsu Photosensor module H7844 (with TE cooling) |
| Transmitted signal registration detector: | Hamamatsu Photosensor module H6780-01 |
| Number of registration channels: | 5 |
| Number of simultaneously registered channels: | up to 5 |
| PC connection interface: | Ethernet 100 Base-T (TCP/IP protocol) |
All components of the system are based on the optical plate with the pneumatic vibrated insulating legs.
