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MICROSCOPE* , model Nikon Ti |
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| Type: | inverted | ||
| Objective lenses: | CFI Plan Fluor 4x, 10x, 20x, 40x, 60x, CF Epi Plan APO 100x | ||
| Stage: | automated | ||
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114 x 75 mm | ||
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0.06 μm / ± 1 μm | ||
| Z-scanner: | piezo scanner | ||
|
80 μm | ||
|
50 nm / < 6 nm | ||
OPTICAL-MECHANICAL UNIT (OMU) |
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| Optimized optics for the spectral range: | 325 - 1100 nm | ||
| Laser radiation delivery: | single, double, triple or penta input port | ||
| Polarizers (excitation and detection channels): | Glan-Taylor prism, 325 - 1000 nm | ||
| Half-wave plate (λ / 2) positioner: | five-position | ||
| Beam expander: | magnification factor 1 - 4 | ||
| Edge filter positioner: | five-position | ||
| Interference filter positioner: | six-position | ||
| OMU and microscope coupling: | three- or five- position switch | ||
| Spatial resolution: | XY: <300 nm, Z: 600 nm (532 nm laser, 100x, NA = 0.9) | ||
IMAGING MONOCHROMATOR-SPECTROGRAPH MSO 5004i |
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| Optical configuration: | vertical | ||
| Focal length: |
520 mm |
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| Ports: | 1 input, 2 output | ||
| Flat field: | 28 x 10 mm | ||
| Grating unit: | 4-position turret | ||
| Grating choice: | 150, 300, 600, 1200, 2400, 3600 l/mm, Echelle 75 l/mm | ||
| Spectral resolution: |
0.25 cm-1 Echelle grating, wavelength 500 nm)
0.9 cm-1 (1800 I/mm grating) |
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| Confocal pinhole: | width 0 - 1.5 mm; step size 0.5 μm | ||
| Wavelength accuracy with CCD camera: | 0.005 nm (1800 I/mm grating) | ||
SCANNING UNIT |
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| Scanning method: | galvanometer scanners of XY mirrors | ||
| Scanning speed: | 3 sec (1001 x 1001 pixels, min. step 20 nm) | ||
| Scanning region: | 150 μm x 150 μm (using 100x objective lens) | ||
CCD CAMERA FOR SPECTROGRAPH |
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| Type: | digital CCD camera HSO 101H | ||
| Sensor: | back-thinned CCD array 2048 x 122 | ||
| Pixel size: | 12 x 12 μm | ||
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Cooling: |
Two-stage Peltier cooling with temperature stabilization to -45 °C | ||
| ADC: | 16 bit | ||
CONFOCAL LASER MICROSCOPE UNIT |
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| Objective positioner: | three-coordinated (XYZ) | ||
| Laser beam attenuator: | VND filter | ||
| Confocal pinhole: | variable from 0 to 1.5 mm, step size 0.5 μm | ||
| Detector: | PMT | ||
LASERS |
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| The system configuration allows of using up to 5 lasers | |||
| Type: | Wavelength, nm |
Power, mW |
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| HeCd laser: | 325 |
10 |
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| HeCd laser: | 441.6 | 50 | |
| DPSS laser: | 473 | 22 | 50 |
| DPSS laser: | 532 | 22 | 50 |
| Helium-neon laser: | 633 | 10 | |
| Semiconductor laser: | 785 | 100 | |